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Protein-Carbohydrate Interactions

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 - Lactose Synthase
     · Enzyme Kinetics


Reference:
Conformation of Carbohydrates
by V.S.R. Rao, P.K. Qasba, P.V. Balaji and R. Chandrasekaran
Conformation of Carbohydrates




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>> Lactose Synthase

Gal-T1    a-Lactalbumin 		Gal-T1 (Galactosyltransferase-1) forms a 1:1 complex with a-Lactalbumin to form Lactose Synthase.

In the presence of a sugar substrate and metal ions, Gal-T1 undergoes a conformation change.

Reaction:
GalT1 alone
  UDP-Gal + GlcNAc » Galb1 - 4GlcNAc

GalT1 with a-Lactalbumin
  UDP-Gal + Glc » Galb1 - 4Glc
Reference:
"Crystal Structure of Lactose Synthase Reveals a Large Conformational Change in its Catalytic Component, the b1,4-Galactosyltransferase-l", B. Ramakrishnan and Pradman K. Qasba: J.Mol. Biol. 2001, v310, p.205-218



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